KlaAtlas · Dataset details

PXD014870

Metabolic regulation of gene expression by histone lactylation

Homo sapiens PRIDE 2019/10

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Dataset overview

Dataset accession

PXD014870

Repository

PRIDE

Publication

31645732

UniProt taxonomy ID

9606

Sample category

Cell line

Tissue / cell type

Human MCF-7 breast cancer cells; acid-extracted histones

Experimental condition / comparison

Three separate SILAC comparisons: DCA (10 mM, 24 h) versus PBS; rotenone (10 nM, 24 h) versus DMSO; hypoxia (1% O2, 24 h) versus normoxia.

Experimental information

Acquisition workflow

Standard

MS platform

Orbitrap

Quantification strategy

SILAC

Kla enrichment method

Pan anti-Kla antibody conjugated to nProtein A Sepharose; histone-peptide immunoprecipitation

Enrichment reagent supplier

PTM Bio Inc. (pan anti-Kla antibody); GE Healthcare BioSciences (nProtein A Sepharose)

Reagent catalogue information

PTM-1401 (pan anti-Kla antibody); nProtein A Sepharose catalogue no. not specified

Data access

MaxQuant parameter files

PXD014870_mqpar.xml

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Raw MS file information

3 processed acquisition files. Original raw data may be accessed through the source repository.

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Show raw MS file names (3 files)

MCF7_DCA_SILAC_Kla_IP.raw
MCF7_Hypoxia_SILAC_Kla_IP.raw
MCF7_Rotenone_SILAC_Kla_IP.raw

Metadata sources

https://doi.org/10.1038/s41586-019-1678-1
https://www.ebi.ac.uk/pride/archive/projects/PXD014870
Methods: Reagents, histone peptide enrichment and SILAC; Extended Data Figs. 2-3

Dataset notes

Sample information and limitations Three different SILAC treatment/control comparisons, one raw acquisition per comparison. Heavy- and light-labeled cells were mixed 1:1 before histone extraction. These are not biological triplicates; independent biological n per comparison is not specified. H/L orientation for the current k_site_positive-derived ratios was not independently recovered, so the follow-up output retains unoriented H/L values.