KlaAtlas ยท Dataset details
PXD019323
Astrocyte-derived lactate aggravates brain injury of ischemic stroke in mice by promoting the formation of protein lactylation
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550
Reidentified Kla sites
303
Reidentified Kla proteins
12
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
10090
Sample category
Animal model
Tissue / cell type
Mouse ischemic/reperfused brain cortex tissue
Experimental condition / comparison
B4/B1: 6 h ischemia vs sham; B7/B1: 1.5 h ischemia + 24 h reperfusion vs sham.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Pan anti-lactyllysine antibody conjugated to Protein A Sepharose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Bio (anti-lactyllysine antibody)
Reagent catalogue information
PTM-1401
Data access
MaxQuant parameter files
PXD019323_mqpar.xml
Raw MS file information
12 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (12 files)
- S9755LPLA_B1_1.raw
- S9755LPLA_B1_2.raw
- S9755LPLA_B1_3.raw
- S9755LPLA_B3_1.raw
- S9755LPLA_B3_2.raw
- S9755LPLA_B3_3.raw
- S9755LPLA_B4_1.raw
- S9755LPLA_B4_2.raw
- S9755LPLA_B4_3.raw
- S9755LPLA_B7_1.raw
- S9755LPLA_B7_2.raw
- S9755LPLA_B7_3.raw
Metadata sources
DOI: 10.7150/thno.96375
PRIDE PXD019323
Evidence E01-E04
PRIDE PXD019323
Evidence E01-E04
Dataset notes
Sample information and limitations
Three sample-level quantitative observations in each published B1, B4 and B7 group. B3 has three submitted raw files but no published condition key. Independent animal counts and pooling are not explicitly documented for each MS sample.
Experimental metadata notes
B4/B1: 6 h ischemia vs sham; B7/B1: 1.5 h ischemia + 24 h reperfusion vs sham. B3 remains unmapped.