KlaAtlas ยท Dataset details
PXD020746
Systematic Analysis of Lysine Lactylation in the Plant Fungal Pathogen Botrytis cinerea
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17
Reidentified Kla sites
10
Reidentified Kla proteins
8
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
332648
Sample category
Fungi
Tissue / cell type
Botrytis cinerea strain B05.10 mycelia
Experimental condition / comparison
Baseline B.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Pan anti-Kla antibody-conjugated agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
Not specified in accessible enrichment protocol
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD020746_mqpar.xml
Raw MS file information
8 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (8 files)
- BC-1.raw
- BC-2.raw
- BC-3.raw
- BC-4.raw
- BC-5.raw
- BC-6.raw
- BC-7.raw
- BC-8.raw
Metadata sources
https://doi.org/10.3389/fmicb.2020.594743
https://www.ebi.ac.uk/pride/archive/projects/PXD020746
Methods: fungal cultivation and affinity enrichment; PRIDE sample protocol
https://www.ebi.ac.uk/pride/archive/projects/PXD020746
Methods: fungal cultivation and affinity enrichment; PRIDE sample protocol
Dataset notes
Sample information and limitations
Protein from four independent biological preparations was pooled. The pooled digest was separated into 80 HPLC fractions and combined into eight fractions. BC-1 to BC-8 are the eight peptide-fraction acquisitions, not eight independent biological replicates.
Experimental metadata notes
Baseline B. cinerea B05.10 lactylome, YEPD culture; no treatment/control MS comparison. Published extraction protocol: 150 rpm, 12 h (repository differs: 180 rpm, 14 h). Lactate-treatment Western blots are separate assays.