KlaAtlas ยท Dataset details

PXD022700

Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii

Toxoplasma gondii PRIDE 2022/10
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109
Reidentified Kla sites
45
Reidentified Kla proteins
12
Raw MS files

Dataset overview

Dataset accession
PXD022700
Repository
PRIDE
Publication
36216028
UniProt taxonomy ID
5811
Sample category
Parasite
Tissue / cell type
Toxoplasma gondii RH-strain tachyzoites purified from the peritoneal fluid of infected mice
Experimental condition / comparison
Baseline global tachyzoite lactylome; no selected treatment/control MS comparison.

Experimental information

Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Pan anti-lactyllysine antibody-conjugated beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM BIO, Hangzhou, China
Reagent catalogue information
PTM-1401

Data access

MaxQuant parameter files
PXD022700_mqpar.xml
Download XML
Raw MS file information
12 processed acquisition files. Original raw data may be accessed through the source repository.
View in PRIDE
Show raw MS file names (12 files)
  • K9184PILa01_1.raw
  • K9184PILa01_2.raw
  • K9184PILa01_3.raw
  • K9184PILa01_4.raw
  • K9184PILa02_1.raw
  • K9184PILa02_2.raw
  • K9184PILa02_3.raw
  • K9184PILa02_4.raw
  • K9184PILa03_1.raw
  • K9184PILa03_2.raw
  • K9184PILa03_3.raw
  • K9184PILa03_4.raw
Metadata sources
https://doi.org/10.1016/j.gpb.2022.09.010
https://www.ebi.ac.uk/pride/archive/projects/PXD022700
Methods: protein extraction and lactylated peptide enrichment
Dataset notes
Sample information and limitations Three biological replicates, each analyzed as four combined peptide fractions: 3 x 4 = 12 raw files. The 01/02/03 component identifies biological replicate and the final _1 to _4 identifies peptide fraction; these are not 12 biological replicates.
Experimental metadata notes Baseline global tachyzoite lactylome; no selected treatment/control MS comparison. Vero-cell culture and high/low glucose experiments described elsewhere in the article are separate functional assays.