KlaAtlas ยท Dataset details
PXD023011
Protein Lactylation Critically Regulates Energy Metabolism in the Protozoan Parasite Trypanosoma brucei
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37
Reidentified Kla sites
14
Reidentified Kla proteins
12
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
185431
Sample category
Parasite
Tissue / cell type
Bloodstream-form Trypanosoma brucei, experimental strain Lister 427, purified from infected BALB/c mouse blood
Experimental condition / comparison
Baseline bloodstream-form parasite lactylome; no selected treatment/control MS comparison.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Antibody beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Biolabs Inc., Chicago, IL, United States
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD023011_mqpar.xml
Raw MS file information
12 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (12 files)
- K9169PILa_Tb_1_1.raw
- K9169PILa_Tb_1_2.raw
- K9169PILa_Tb_1_3.raw
- K9169PILa_Tb_1_4.raw
- K9169PILa_Tb_2_1.raw
- K9169PILa_Tb_2_2.raw
- K9169PILa_Tb_2_3.raw
- K9169PILa_Tb_2_4.raw
- K9169PILa_Tb_3_1.raw
- K9169PILa_Tb_3_2.raw
- K9169PILa_Tb_3_3.raw
- K9169PILa_Tb_3_4.raw
Metadata sources
https://doi.org/10.3389/fcell.2021.719720
https://www.ebi.ac.uk/pride/archive/projects/PXD023011
Methods: parasite preparation and affinity enrichment
https://www.ebi.ac.uk/pride/archive/projects/PXD023011
Methods: parasite preparation and affinity enrichment
Dataset notes
Sample information and limitations
Three biological replicates, each separated into four combined peptide fractions: 3 x 4 = 12 selected raw files. In K9169PILa_Tb_r_f, r=1-3 denotes replicate and f=1-4 denotes fraction.
Experimental metadata notes
Baseline bloodstream-form parasite lactylome; no selected treatment/control MS comparison. Glucose, 2-deoxyglucose and oxamate interventions are separate functional assays. Strain 927/4 GUTat10.1 is the search reference database, not the stated experimental strain.