KlaAtlas ยท Dataset details
PXD028488
Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome
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N/A
Reidentified Kla sites
N/A
Reidentified Kla proteins
11
Raw MS files
Dataset overview
Dataset accession
PXD028488
Repository
iProX
Publication
UniProt taxonomy ID
9606;10090
Sample category
Cell line
Tissue / cell type
Human HEK293T, HCT116 and TALL-104 cells; mouse BV2 microglial and RAW264.7 macrophage cells
Experimental condition / comparison
Enriched cellular lactylproteomes after 25 mM lactate for 24 h; HEK293T measurements benchmark HCD collision energies 24/28/32/36/40.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Anti-lactylation antibody conjugated to Protein A agarose; peptide affinity enrichment
Enrichment reagent supplier
Invitrogen (Protein A agarose); anti-lactylation antibody supplier not specified
Reagent catalogue information
15918014 (Protein A agarose); anti-lactylation antibody catalogue no. not specified
Data access
MaxQuant parameter files
PXD028488_human_mqpar.xml, PXD028488_mouse_mqpar.xml
Raw MS file information
11 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (11 files)
- 20211123-293T-lac-enrich-mix-HCD24.raw
- 20211123-293T-lac-enrich-mix-HCD28.raw
- 20211123-293T-lac-enrich-mix-HCD32.raw
- 20211123-293T-lac-enrich-mix-HCD36.raw
- 20211123-293T-lac-enrich-mix-HCD40.raw
- 20211127-HCT116-LAC-Enrichment-1.raw
- TALL-NaLAC-enrichment-1.raw
- TALL-NaLAC-enrichment-2.raw
- TALL-NaLAC-enrichment-3.raw
- 20211215-BV2-Enrichment-HCD36.raw
- 20211215-RAW-Enrichment-HCD36.raw
Metadata sources
https://doi.org/10.1038/s41592-022-01523-1
https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD028488
Supplementary Fig. 3; Supplementary Tables 2 and 4; Reporting Summary (including supplementary information)
https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD028488
Supplementary Fig. 3; Supplementary Tables 2 and 4; Reporting Summary (including supplementary information)
Dataset notes
Sample information and limitations
11 acquisitions: five HEK293T HCD collision-energy settings of an enriched mixture (24/28/32/36/40), one HCT116, three TALL-104, one BV2 and one RAW264.7. HEK293T settings are not biological replicates. Biological versus technical independence of TALL-104 files 1-3 is unspecified.
Experimental metadata notes
Enriched cellular lactylproteomes after 25 mM lactate for 24 h; HEK293T measurements benchmark HCD collision energies 24/28/32/36/40. All 11 selected files are enriched lactylproteomes; no untreated-control acquisition is selected.