KlaAtlas ยท Dataset details
PXD028737
YY1 lactylation in microglia promotes angiogenesis through transcription activation-mediated upregulation of FGF2
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2,719
Reidentified Kla sites
1,178
Reidentified Kla proteins
4
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human HMC3 microglial cell line
Experimental condition / comparison
Hypoxia (1% O2, 24 h) vs normoxia (24 h)
Experimental information
Acquisition workflow
TIMS-DDA
MS platform
Bruker TIMS
Quantification strategy
Label-free
Kla enrichment method
PTM Bio pre-washed pan-antibody beads; Kla-peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Bio
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD028737_mqpar.xml
Raw MS file information
4 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (4 files)
- H0_Slot1-35_1_10128.d
- H0_Slot2-51_1_10185.d
- H24_Slot1-34_1_10126.d
- H24_Slot2-50_1_10183.d
Metadata sources
DOI: 10.1186/s13059-023-02931-y
PRIDE PXD028737
Evidence E01-E04
PRIDE PXD028737
Evidence E01-E04
Dataset notes
Sample information and limitations
Selected four files comprise two assays: one lactylome acquisition per condition (Slot1) plus one total-proteome acquisition per condition (Slot2). These are not two Kla replicates per condition. Directional HMC3/ACHN cross-cell-line comparison is descriptive only: HMC3 has one selected Kla acquisition per group and ACHN biological independence is not established.