KlaAtlas ยท Dataset details
PXD030345
YiaC and CobB regulate lysine lactylation in Escherichia coli
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1,564
Reidentified Kla sites
743
Reidentified Kla proteins
3
Raw MS files
Dataset overview
Dataset accession
PXD030345
Repository
iProX
Publication
UniProt taxonomy ID
83333
Sample category
Bacteria
Tissue / cell type
Escherichia coli K-12 MG1655 whole-cell lysate
Experimental condition / comparison
delta cobB vs WT.
Experimental information
Acquisition workflow
FAIMS
MS platform
Orbitrap
Quantification strategy
SILAC
Kla enrichment method
Anti-lactyllysine antibody-conjugated Protein A agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Biolabs
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD030345_mqpar.xml
Raw MS file information
3 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (3 files)
- 20210626_LCC_DHY_La_FS-40.raw
- 20210626_LCC_DHY_La_FS-60.raw
- 20210626_LCC_DHY_La_FS-80.raw
Metadata sources
DOI: 10.1038/s41467-022-34399-y
PRIDE PXD030345
Evidence E01-E04
PRIDE PXD030345
Evidence E01-E04
Dataset notes
Sample information and limitations
Metabolic SILAC WT/KO mixed 1:1; three selected RAW acquisitions. Published site table contains one aggregate H/L comparison and no independent replicate series. Biological n cannot be inferred from the three files or their 40/60/80 suffixes.
Experimental metadata notes
delta cobB vs WT. Heavy=WT, light=delta cobB; original H/L=WT/KO. For KO/WT log2 response use -log2(H/L).