KlaAtlas ยท Dataset details
PXD030346
YiaC and CobB regulate lysine lactylation in Escherichia coli
Back to datasets
955
Reidentified Kla sites
528
Reidentified Kla proteins
3
Raw MS files
Dataset overview
Dataset accession
PXD030346
Repository
iProX
Publication
UniProt taxonomy ID
83333
Sample category
Bacteria
Tissue / cell type
Escherichia coli K-12 MG1655 whole-cell lysate
Experimental condition / comparison
delta yiaC vs WT.
Experimental information
Acquisition workflow
FAIMS
MS platform
Orbitrap
Quantification strategy
SILAC
Kla enrichment method
Anti-lactyllysine antibody-conjugated Protein A agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Biolabs
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD030346_mqpar.xml
Raw MS file information
3 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (3 files)
- 20210425_LCC_ZK_Elute_40V.raw
- 20210425_LCC_ZK_Elute_60V.raw
- 20210425_LCC_ZK_Elute_80V.raw
Metadata sources
DOI: 10.1038/s41467-022-34399-y
PRIDE PXD030346
Evidence E01-E04
PRIDE PXD030346
Evidence E01-E04
Dataset notes
Sample information and limitations
Metabolic SILAC WT/KO mixed 1:1; three selected RAW acquisitions. Published site table contains one aggregate H/L comparison and no independent replicate series. Biological n cannot be inferred from the three files or their 40/60/80 suffixes.
Experimental metadata notes
delta yiaC vs WT. Heavy=delta yiaC; light=yiaC WT. H/L=KO/WT. For KO/WT log2 response use log2(H/L).