KlaAtlas ยท Dataset details
PXD033146
Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy
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1,624
Reidentified Kla sites
537
Reidentified Kla proteins
4
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
9606
Sample category
Disease tissue
Tissue / cell type
Human rotator cuff tendinopathy lesion tendon and adjacent unaffected tendon from nine patients (paired tissue collection)
Experimental condition / comparison
RCT lesion group pool versus adjacent unaffected tendon group pool
Experimental information
Acquisition workflow
Reporter MS2
MS platform
Orbitrap
Quantification strategy
TMT
Kla enrichment method
Anti-L-lactyllysine antibody-conjugated agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Bio
Reagent catalogue information
PTM-1404
Data access
MaxQuant parameter files
PXD033146_mqpar.xml
Raw MS file information
4 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (4 files)
- HA119TPLa_Fr1.raw
- HA119TPLa_Fr2.raw
- HA119TPLa_Fr3.raw
- HA119TPLa_Fr4.raw
Metadata sources
DOI: 10.1021/acs.jproteome.2c00756
PRIDE PXD033146
Evidence E01-E05
PRIDE PXD033146
Evidence E01-E05
Dataset notes
Sample information and limitations
Nine paired patients were pooled by group; each pool analyzed three times. Table S3 has RCT1-3 and NC1-3, representing repeated analyses of pooled material, not independent patient replicates. Four RAW files are fractions of one TMT-6plex multiplex. Reporter-mass-to-RCT/NC mapping remains unresolved; the pooled analyses do not provide patient-level biological variance.