KlaAtlas ยท Dataset details

PXD033454

A bioorthogonal chemical reporter for the detection and identification of protein lactylation

Homo sapiens PRIDE 2022/04
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215
Reidentified Kla sites
125
Reidentified Kla proteins
16
Raw MS files

Dataset overview

Dataset accession
PXD033454
Repository
PRIDE
Publication
35685793
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human HEK293T cells, separately analysed nuclear and nonnuclear/cytoplasmic fractions.
Experimental condition / comparison
YnLac metabolic-probe labelling versus L-lactate-treated control in both nuclear and nonnuclear fractions.

Experimental information

Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
YnLac chemical-reporter labeling, CuAAC with acid-cleavable azido-DADPS-biotin, then streptavidin-agarose affinity capture
Enrichment reagent supplier
Click Chemistry Tools (azido-DADPS-biotin); ThermoFisher (streptavidin agarose)
Reagent catalogue information
1330 (acid-cleavable azido-DADPS-biotin); streptavidin agarose catalogue no. not specified

Data access

MaxQuant parameter files
PXD033454_mqpar.xml
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Raw MS file information
16 processed acquisition files. Original raw data may be accessed through the source repository.
View in PRIDE
Show raw MS file names (16 files)
  • NonNucLB1.raw
  • NonNucLB2.raw
  • NonNucLB3.raw
  • NonNucLB4.raw
  • NonNucPB1.raw
  • NonNucPB2.raw
  • NonNucPB3.raw
  • NonNucPB4.raw
  • NucLB1.raw
  • NucLB2.raw
  • NucLB3.raw
  • NucLB4.raw
  • NucPB1.raw
  • NucPB2.raw
  • NucPB3.raw
  • NucPB4.raw
Metadata sources
https://doi.org/10.1039/d2sc00918h
https://www.ebi.ac.uk/pride/archive/projects/PXD033454
Results: Chemical proteomic profiling of lactylation using YnLac; Fig. 3
Dataset notes
Sample information and limitations Four biological replicates per treatment, with separate nuclear and nonnuclear fractions: 2 treatments x 4 biological replicates x 2 subcellular fractions = 16 selected raw files. Subcellular fractions are not additional independent biological replicates.
Experimental metadata notes YnLac metabolic-probe labelling versus L-lactate-treated control in both nuclear and nonnuclear fractions. LB files are YnLac-labelled; PB files are L-lactate controls (as explicitly defined in Supplementary Tables S2/S4).