KlaAtlas ยท Dataset details
PXD033991
Identification of lysine-lactylated substrates in gastric cancer cells
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2,952
Reidentified Kla sites
1,228
Reidentified Kla proteins
1
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human AGS gastric adenocarcinoma cells.
Experimental condition / comparison
Baseline global lysine-lactylome profiling of AGS cells; the selected MS file does not encode a lactate-versus-vehicle comparison.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Anti-L-lactyllysine antibody-conjugated agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
Micron Biotechnology Company
Reagent catalogue information
WM102
Data access
MaxQuant parameter files
PXD033991_mqpar.xml
Raw MS file information
1 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (1 files)
- QE_HFX_20210524_50cm_60min_IP.raw
Metadata sources
https://doi.org/10.1016/j.isci.2022.104630
https://www.ebi.ac.uk/pride/archive/projects/PXD033991
STAR Methods: key resources table and LC-MS/MS analysis
https://www.ebi.ac.uk/pride/archive/projects/PXD033991
STAR Methods: key resources table and LC-MS/MS analysis
Dataset notes
Sample information and limitations
One selected LC-MS/MS acquisition from three independently cultured AGS replicates harvested and mixed before MS sample preparation; pooled biological material, not three independently quantified MS replicates.
Experimental metadata notes
Baseline global lysine-lactylome profiling of AGS cells; the selected MS file does not encode a lactate-versus-vehicle comparison. The paper's lactate treatment comparisons belong to separate RNA-seq and validation experiments.