KlaAtlas ยท Dataset details
PXD037371
Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma
Back to datasets
2,565
Reidentified Kla sites
1,156
Reidentified Kla proteins
10
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
9606
Sample category
Disease tissue
Tissue / cell type
Human normal liver, primary hepatocellular carcinoma without metastasis during 3-year follow-up, and lung metastases from hepatocellular carcinoma.
Experimental condition / comparison
Three tissue groups: normal liver versus nonmetastatic primary HCC versus HCC lung metastases; TMT-labelled comparative lactylome.
Experimental information
Acquisition workflow
Reporter MS2
MS platform
Orbitrap
Quantification strategy
TMT
Kla enrichment method
Pan anti-Kla antibody-conjugated agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
Not specified in accessible enrichment protocol
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD037371_mqpar.xml
Raw MS file information
10 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (10 files)
- QE_HFX_20211228_50cm_60min_01.raw
- QE_HFX_20211228_50cm_60min_02.raw
- QE_HFX_20211228_50cm_60min_03.raw
- QE_HFX_20211228_50cm_60min_04.raw
- QE_HFX_20211228_50cm_60min_05.raw
- QE_HFX_20211228_50cm_60min_06.raw
- QE_HFX_20211228_50cm_60min_07.raw
- QE_HFX_20211228_50cm_60min_08.raw
- QE_HFX_20211228_50cm_60min_09.raw
- QE_HFX_20211228_50cm_60min_10.raw
Metadata sources
https://doi.org/10.1002/pmic.202200432
https://www.ebi.ac.uk/pride/archive/projects/PXD037371
Abstract; PRIDE project description and affinity enrichment protocol
User-shared working sample map (conditional; not independently confirmed in primary archive): https://chatgpt.com/share/6adee875-f0e4-83e9-9d7e-5d6a2c369442
https://www.ebi.ac.uk/pride/archive/projects/PXD037371
Abstract; PRIDE project description and affinity enrichment protocol
User-shared working sample map (conditional; not independently confirmed in primary archive): https://chatgpt.com/share/6adee875-f0e4-83e9-9d7e-5d6a2c369442
Dataset notes
Sample information and limitations
Article reports nine tissue samples, three per group. Ten selected RAWs are HPLC peptide fractions of one TMT experiment. The working reporter map used for conditional analysis is user-shared and not independently confirmed in PRIDE: 126C/127N/127C=normal liver; 128N=reference/QC and excluded; 128C/129N/129C=lung metastasis; 130N/130C/131N=primary HCC. Ten fractions were summed within each Site ID and channel; fractions are not biological replicates. The three mapped reporter channels per tissue group are conditional sample units pending the primary reporter-to-sample key.
Experimental metadata notes
Three tissue groups: normal liver versus nonmetastatic primary HCC versus HCC lung metastases; TMT-labelled comparative lactylome. Separate exploratory comparison: lung metastasis versus primary HCC; do not pool this effect with tumor-versus-normal comparisons.