KlaAtlas ยท Dataset details
PXD040840
Lysine L-lactylation is the dominant lactylation isomer induced by glycolysis
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187
Reidentified Kla sites
68
Reidentified Kla proteins
10
Raw MS files
Dataset overview
Dataset accession
PXD040840
Repository
iProX
Publication
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human MCF-7 breast cancer cells; selected SILAC quantification is acid-extracted histones
Experimental condition / comparison
Selected histone SILAC: 25 mM versus 0 mM glucose for 24 h, heavy=25 mM and light=0 mM; high/no-glucose response=H/L.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free;SILAC
Kla enrichment method
nProtein A Sepharose antibody affinity enrichment of KL-la/KD-la/Kce peptides
Enrichment reagent supplier
Cytiva (nProtein A Sepharose); antibody supplier not specified in accessible peptide-enrichment protocol
Reagent catalogue information
Not specified for enrichment
Data access
MaxQuant parameter files
PXD040840_label-free_mqpar.xml, PXD040840_silac_mqpar.xml
Raw MS file information
10 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (10 files)
- Kce_IP_R1.raw
- Kce_IP_R2.raw
- Kce_IP_R3.raw
- KD-la_IP_R1.raw
- KD-la_IP_R2.raw
- KD-la_IP_R3.raw
- KL-la_IP_R1.raw
- KL-la_IP_R2.raw
- KL-la_IP_R3.raw
- KL-la_IP_SILAC_high_low_glucose.raw
Metadata sources
https://doi.org/10.1038/s41589-024-01680-8
Methods: Reagents and Peptide enrichment; Fig. 4; Extended Data Fig. 7
Methods: Reagents and Peptide enrichment; Fig. 4; Extended Data Fig. 7
Dataset notes
Sample information and limitations
One selected histone SILAC acquisition, H=25 mM glucose and L=0 mM glucose, mixed 1:1; independent biological n unspecified. Nine LFQ acquisitions are three enrichment classes with R1-R3 each, with biological/technical independence unspecified; they do not establish glucose-response replicates.
Experimental metadata notes
Selected histone SILAC: 25 mM versus 0 mM glucose for 24 h, heavy=25 mM and light=0 mM; high/no-glucose response=H/L. Nine other label-free files represent Kce, KD-la and KL-la enrichment classes, not glucose treatment groups. A separate whole-cell comparison used 0.1 versus 25 mM glucose.