KlaAtlas ยท Dataset details
PXD042171
Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses
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2,681
Reidentified Kla sites
1,077
Reidentified Kla proteins
6
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
10090
Sample category
Primary cell
Tissue / cell type
Mouse macrophages; dedicated Table S1 identifies peritoneal macrophages, but Fig. 4i identifies BMDMs. Exact source of selected RAWs unresolved.
Experimental condition / comparison
VSV-associated comparison group V versus medium-alone control C; C1-C3 and V1-V3 identify six quantitative samples.
Experimental information
Acquisition workflow
TIMS-DDA
MS platform
Bruker TIMS
Quantification strategy
Label-free
Kla enrichment method
Anti-lactyllysine antibody-conjugated agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
PTM Bio
Reagent catalogue information
PTM-1404
Data access
MaxQuant parameter files
PXD042171_mqpar.xml
Raw MS file information
6 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (6 files)
- FE139LPLaB1_C1_Slot2-5_1_21433.d
- FE139LPLaB1_C2_Slot2-6_1_21435.d
- FE139LPLaB1_C3_Slot2-7_1_21437.d
- FE139LPLaB1_V1_Slot2-8_1_21439.d
- FE139LPLaB1_V2_Slot2-9_1_21441.d
- FE139LPLaB1_V3_Slot2-10_1_21443.d
Metadata sources
https://doi.org/10.1038/s41422-024-01060-w
https://www.ebi.ac.uk/pride/archive/projects/PXD042171
Methods: lactylome sample preparation and affinity enrichment
https://www.ebi.ac.uk/pride/archive/projects/PXD042171
Methods: lactylome sample preparation and affinity enrichment
Dataset notes
Sample information and limitations
Downloaded dedicated lactylome Table S1 explicitly contains C1-C3 and V1-V3; relevant figure legends report n=3. Independent source animals, preparations and any pooling behind these numbered MS samples are not explicitly defined.