KlaAtlas ยท Dataset details

PXD046488

Global Analysis of Lysine Lactylation of Germinated Seeds in Wheat

Triticum aestivum PRIDE 2023/11
Back to datasets
188
Reidentified Kla sites
80
Reidentified Kla proteins
10
Raw MS files

Dataset overview

Dataset accession
PXD046488
Repository
PRIDE
Publication
38003390
UniProt taxonomy ID
4565
Sample category
Plant
Tissue / cell type
Embryos of common wheat (Triticum aestivum L.), cultivar QiMin23
Experimental condition / comparison
0 h versus 12 h after imbibition; seeds imbibed in darkness at 21 degrees C.

Experimental information

Acquisition workflow
Reporter MS2
MS platform
Orbitrap
Quantification strategy
TMT
Kla enrichment method
Pre-washed anti-lactyllysine antibody-conjugated agarose beads; peptide immunoaffinity enrichment
Enrichment reagent supplier
Micrometer Biotech
Reagent catalogue information
Not specified in accessible enrichment protocol

Data access

MaxQuant parameter files
PXD046488_mqpar.xml
Download XML
Raw MS file information
10 processed acquisition files. Original raw data may be accessed through the source repository.
View in PRIDE
Show raw MS file names (10 files)
  • 1.raw
  • 2.raw
  • 3.raw
  • 4.raw
  • 5.raw
  • 6.raw
  • 7.raw
  • 8.raw
  • 9.raw
  • 10.raw
Metadata sources
https://doi.org/10.3390/ijms242216195
https://www.ebi.ac.uk/pride/archive/projects/PXD046488
Methods: plant materials and affinity enrichment
Dataset notes
Sample information and limitations Three biological replicates per time point (six samples total), TMT-labeled and pooled after labeling. The ten raws (1.raw-10.raw) are configured as peptide fractions 1-10 of experiment Ta in mqpar, not ten biological replicates.