KlaAtlas ยท Dataset details
PXD048127
Genetic code expansion reveals site-specific lactylation in living cells reshapes protein functions
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N/A
Reidentified Kla sites
N/A
Reidentified Kla proteins
7
Raw MS files
Dataset overview
Dataset accession
PXD048127
Repository
iProX
Publication
UniProt taxonomy ID
9606;10090
Sample category
Cell line
Tissue / cell type
Seven selected tumor-derived cell lines: human A549, H1299, HepG2, PC3 and THP1; mouse LLC and MC38
Experimental condition / comparison
Lactylproteome survey after 25 mM sodium lactate for 24 h; affinity-enriched lactylated peptides.
Experimental information
Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Anti-lactylation antibody conjugated to Protein A agarose; peptide immunoprecipitation enrichment
Enrichment reagent supplier
Anti-lactylation antibody source not reported; Protein A agarose: Invitrogen
Reagent catalogue information
Antibody not reported; Protein A agarose: 15918014
Data access
MaxQuant parameter files
PXD048127_human_mqpar.xml, PXD048127_mouse_mqpar.xml
Raw MS file information
7 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (7 files)
- A549-Enrichment.raw
- H1299-Enrichment.raw
- HepG2-Enrichment.raw
- PC3-Enrichment.raw
- THP1-Enrichment.raw
- LLC-Enrichment.raw
- MC38-Enrichment.raw
Metadata sources
https://doi.org/10.1038/s41467-024-55165-2
Methods: Cell culture; Wet-lab strategy for lactylation mining; Fig. 1b (including supplementary information)
Methods: Cell culture; Wet-lab strategy for lactylation mining; Fig. 1b (including supplementary information)
Dataset notes
Sample information and limitations
One selected enriched-lactylome acquisition per cell line, seven raws total. Independent biological replicate count is unspecified for this survey. The other cell lines and later functional experiments in the article are outside the selected file set.
Experimental metadata notes
Lactylproteome survey after 25 mM sodium lactate for 24 h; affinity-enriched lactylated peptides. No untreated-comparator raw file is selected.