KlaAtlas ยท Dataset details
PXD048244
Systematic analysis of lysine lactylation in nucleus pulposus cells
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4,363
Reidentified Kla sites
1,181
Reidentified Kla proteins
6
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
10116
Sample category
Primary cell
Tissue / cell type
Primary rat nucleus pulposus cells isolated from central nucleus pulposus tissue of intervertebral discs from 8-week-old male Sprague-Dawley rats (~220 g).
Experimental condition / comparison
NC: normoxia (21% O2), 24 h; HY: hypoxia (1% O2), 24 h.
Experimental information
Acquisition workflow
TIMS-DDA
MS platform
Bruker TIMS
Quantification strategy
Label-free
Kla enrichment method
Pre-washed antibody-bead immunoaffinity enrichment of Kla-modified peptides
Enrichment reagent supplier
PTM Bio Inc.
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD048244_mqpar.xml
Raw MS file information
6 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (6 files)
- AE141LPLa_HY1_Slot2-17_1_10726.d
- AE141LPLa_HY2_Slot2-18_1_10728.d
- AE141LPLa_HY3_Slot2-19_1_10730.d
- AE141LPLa_NC1_Slot2-14_1_10720.d
- AE141LPLa_NC2_Slot2-15_1_10722.d
- AE141LPLa_NC3_Slot2-16_1_10724.d
Metadata sources
DOI: 10.1016/j.isci.2024.111157
PRIDE PXD048244
Evidence E01-E04
PRIDE PXD048244
Evidence E01-E04
Dataset notes
Sample information and limitations
3 submitted cell samples/condition (HY1-3, NC1-3), one selected TIMS-DDA acquisition/sample, fraction=1. The MS method explicitly reports 3 samples/group; independent donor/isolation/culture replicates and pooling are not described. Do not transfer n=3 biological-replicate wording from Western-blot validation to the MS samples.
Experimental metadata notes
NC: normoxia (21% O2), 24 h; HY: hypoxia (1% O2), 24 h. These cultured-cell oxygen treatments model different NP oxygen environments; the MS samples are not human IDD tissue.