KlaAtlas ยท Dataset details
PXD050906
NBS1 lactylation is required for efficient DNA repair and chemotherapy resistance
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4,822
Reidentified Kla sites
1,902
Reidentified Kla proteins
6
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human AGS gastric cancer cells: parental AGS-P and acquired cisplatin-resistant AGS-R
Experimental condition / comparison
Acquired cisplatin-resistant AGS-R (Treat_1-3) versus parental AGS-P (Ctrl_1-3); mapping confirmed by complete numerical matching to Supplementary Table 3.
Experimental information
Acquisition workflow
TIMS-DDA
MS platform
Bruker TIMS
Quantification strategy
Label-free
Kla enrichment method
Pre-washed antibody-bead affinity enrichment of lactyl-modified peptides
Enrichment reagent supplier
Not specified in accessible enrichment protocol
Reagent catalogue information
Not specified in accessible enrichment protocol
Data access
MaxQuant parameter files
PXD050906_mqpar.xml
Raw MS file information
6 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (6 files)
- KA460LPLa_Ctrl_1_Slot1-38_1_9994.d
- KA460LPLa_Ctrl_2_Slot1-38_1_9996.d
- KA460LPLa_Ctrl_3_Slot1-38_1_9998.d
- KA460LPLa_Treat_1_Slot2-54_1_10072.d
- KA460LPLa_Treat_2_Slot2-54_1_10074.d
- KA460LPLa_Treat_3_Slot2-54_1_10076.d
Metadata sources
https://doi.org/10.1038/s41586-024-07620-9
https://www.ebi.ac.uk/pride/archive/projects/PXD050906
Methods: cell models and lactylated peptide enrichment
https://www.ebi.ac.uk/pride/archive/projects/PXD050906
Methods: cell models and lactylated peptide enrichment
Dataset notes
Sample information and limitations
Six acquisitions, three per arm: Ctrl_1-3=parental AGS-P; Treat_1-3=cisplatin-resistant AGS-R. Independent cultures/preparations versus technical repeats remain unresolved. Matching numeric suffixes do not establish paired cultures. The AGS effect is therefore retained as descriptive until biological independence is confirmed.