KlaAtlas ยท Dataset details

PXD051415

HBO1 catalyzes lysine lactylation and mediates histone H3K9la to regulate gene transcription

Homo sapiens PRIDE 2024/04
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418
Reidentified Kla sites
260
Reidentified Kla proteins
9
Raw MS files

Dataset overview

Dataset accession
PXD051415
Repository
PRIDE
Publication
38670996
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human HeLa cervical cancer cells; histone and whole-cell lactylation samples
Experimental condition / comparison
HBO1-knockout versus WT HeLa cells.

Experimental information

Acquisition workflow
FAIMS
MS platform
Orbitrap
Quantification strategy
SILAC
Kla enrichment method
Anti-L-lactyllysine antibody-conjugated Protein A agarose immunoaffinity enrichment of peptides
Enrichment reagent supplier
PTM Biolabs
Reagent catalogue information
Not specified in accessible enrichment protocol

Data access

MaxQuant parameter files
PXD051415_mqpar.xml
Download XML
Raw MS file information
9 processed acquisition files. Original raw data may be accessed through the source repository.
View in PRIDE
Show raw MS file names (9 files)
  • HeLa-Histone-La.raw
  • HeLa-Kla-1.raw
  • HeLa-Kla-2.raw
  • HeLa-Kla-3.raw
  • HeLa-Kla-4.raw
  • HeLa-Kla-5.raw
  • HeLa-Kla-6.raw
  • HeLa-Kla-7.raw
  • HeLa-Kla-8.raw
Metadata sources
https://doi.org/10.1038/s41467-024-47900-6
https://www.ebi.ac.uk/pride/archive/projects/PXD051415
Methods: SILAC quantification and lactylated peptide enrichment
Dataset notes
Sample information and limitations One histone SILAC acquisition and eight whole-cell Kla acquisitions configured as fractions 1-8 of one HeLa experiment in mqpar. Original offline fraction preparation and independent biological n remain unspecified. Both workflows use FAIMS -45/-65 V; eight files are not eight biological replicates.
Experimental metadata notes HBO1-knockout versus WT HeLa cells. SILAC heavy=WT and light=HBO1-KO, mixed 1:1; orient KO/WT as L/H. Histone and whole-cell preparations must be analyzed separately.