KlaAtlas ยท Dataset details
PXD053029
YTHDC1 lactylation regulates its phase separation to enhance target mRNA stability and promote RCC progression
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2,148
Reidentified Kla sites
1,030
Reidentified Kla proteins
6
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human ACHN renal cell carcinoma cells
Experimental condition / comparison
ACHN hypoxia for 24 h (H24_1-3) versus normoxia (C_1-3).
Experimental information
Acquisition workflow
TIMS-DDA
MS platform
Bruker TIMS
Quantification strategy
Label-free
Kla enrichment method
Pre-washed antibody-bead enrichment of modified peptides for lactylome analysis
Enrichment reagent supplier
PTM Bio
Reagent catalogue information
Not specified; the source reports lot number 001
Data access
MaxQuant parameter files
PXD053029_mqpar.xml
Raw MS file information
6 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (6 files)
- C_1_Slot2-20_1_5362.d
- C_2_Slot2-20_1_5363.d
- C_3_Slot2-20_1_5364.d
- H24_1_Slot2-21_1_5366.d
- H24_2_Slot2-21_1_5367.d
- H24_3_Slot2-21_1_5368.d
Metadata sources
https://doi.org/10.1016/j.molcel.2025.06.017
https://www.ebi.ac.uk/pride/archive/projects/PXD053029
Abstract; PRIDE project description and sample protocol
https://www.ebi.ac.uk/pride/archive/projects/PXD053029
Abstract; PRIDE project description and sample protocol
Dataset notes
Sample information and limitations
C_1-3 and H24_1-3: six acquisitions. Independent biological replication versus technical repeats is not explicitly established for these selected files. The ACHN-HMC3 hypoxia overlap is descriptive only; do not treat acquisition counts as confirmed biological n.
Experimental metadata notes
ACHN hypoxia for 24 h (H24_1-3) versus normoxia (C_1-3). Original processed parameters/summary confirm PXD053029 is the lactylome; the article resource-table proteome/lactylome accessions are swapped.