KlaAtlas ยท Dataset details
PXD053276
Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue
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1,317
Reidentified Kla sites
261
Reidentified Kla proteins
6
Raw MS files
Dataset overview
Dataset accession
Repository
PRIDE
Publication
UniProt taxonomy ID
10090
Sample category
Animal model
Tissue / cell type
Mouse hind-leg skeletal muscle; 8-week-old male C57BL/6J mice, approximately 21 +/- 1 g at enrollment.
Experimental condition / comparison
Con: sedentary control; EX: moderate treadmill exercise 60 min/day, 5 days/week, for 6 weeks, after a 5-day adaptation; tissue collected 24 h after final training.
Experimental information
Acquisition workflow
TIMS-DDA
MS platform
Bruker TIMS
Quantification strategy
Label-free
Kla enrichment method
PTM-1404 resin immunoaffinity enrichment of lactylated peptides
Enrichment reagent supplier
PTM Bio
Reagent catalogue information
PTM-1404
Data access
MaxQuant parameter files
PXD053276_mqpar.xml
Raw MS file information
6 processed acquisition files.
Original raw data may be accessed
through the source repository.
Show raw MS file names (6 files)
- XA04524B2LPLa_Con_1_Slot2-7_1_21842.d
- XA04524B2LPLa_Con_2_Slot2-8_1_21844.d
- XA04524B2LPLa_Con_3_Slot2-9_1_21846.d
- XA04524B2LPLa_EX_1_Slot2-10_1_21848.d
- XA04524B2LPLa_EX_2_Slot2-11_1_21850.d
- XA04524B2LPLa_EX_3_Slot2-12_1_21852.d
Metadata sources
DOI: 10.3389/fcell.2024.1472338
PRIDE PXD053276
Evidence E01-E04
PRIDE PXD053276
Evidence E01-E04
Dataset notes
Sample information and limitations
3 quantitative muscle samples/group (Con_1-3, EX_1-3), 1 selected TIMS-DDA acquisition/sample, fraction=1; Con=sedentary and EX=6-week treadmill training, collection 24 h after final training. Table 3 confirms 3 columns/group; individual-mouse provenance and pooling remain unreported, so independent biological n is unresolved.