KlaAtlas ยท Dataset details

PXD053474

Subcellular Proteomic Mapping of Lysine Lactylation

Homo sapiens PRIDE 2024/12
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364
Reidentified Kla sites
226
Reidentified Kla proteins
19
Raw MS files

Dataset overview

Dataset accession
PXD053474
Repository
PRIDE
Publication
39569522
UniProt taxonomy ID
9606
Sample category
Cell line
Tissue / cell type
Human HCT116 colon carcinoma cells; whole-cell, cytoplasmic, nuclear, mitochondrial and membrane fractions
Experimental condition / comparison
Sodium-lactate-treated HCT116 cells (25 mM, 48 h); subcellular and whole-cell lactylome coverage.

Experimental information

Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Anti-Kla antibody conjugated to Protein A/G Plus agarose for lactylated-peptide affinity enrichment
Enrichment reagent supplier
PTM Bio Inc. (anti-Kla antibody); Santa Cruz Biotechnology (Protein A/G Plus agarose)
Reagent catalogue information
PTM-1401RM (anti-Kla antibody); sc-2003 (Protein A/G Plus agarose)

Data access

MaxQuant parameter files
PXD053474_DDA_mqpar.xml
Download XML
Raw MS file information
19 processed acquisition files. Original raw data may be accessed through the source repository.
View in PRIDE
Show raw MS file names (19 files)
  • 20240110-Whole-cell-Lactylation-F1.raw
  • 20240110-Whole-cell-Lactylation-F1-2.raw
  • 20240110-Whole-cell-Lactylation-F2.raw
  • 20240110-Whole-cell-Lactylation-F3.raw
  • 20240110-Whole-cell-Lactylation-NoFrac.raw
  • 20240111-Cytoplasm-Lactylation-F1.raw
  • 20240111-Cytoplasm-Lactylation-F2.raw
  • 20240111-Cytoplasm-Lactylation-F3.raw
  • 20240111-Cytoplasm-Lactylation-F4.raw
  • 20240112-Nucleus-Lactylation-F1.raw
  • 20240112-Nucleus-Lactylation-F2.raw
  • 20240112-Nucleus-Lactylation-F3.raw
  • 20240112-Nucleus-Lactylation-F4.raw
  • 20240114-Mitochondria-Lactylation-F1.raw
  • 20240114-Mitochondria-Lactylation-F2.raw
  • 20240114-Mitochondria-Lactylation-F3.raw
  • 20240114-Mitochondria-Lactylation-F4.raw
  • 20240116-Membrane-Lactylation-F1.raw
  • 20240116-Membrane-Lactylation-F2.raw
Metadata sources
https://doi.org/10.1021/jasms.4c00366
https://www.ebi.ac.uk/pride/archive/projects/PXD053474
Abstract; PRIDE project description and sample protocol
Dataset notes
Sample information and limitations 19 acquisitions: four configured WholeCell fractions (filenames F1/F1-2/F2/F3; mqpar fractions 1-4), separate WholeCellNoFrac, four fractions each for cytoplasm/nucleus/mitochondria, and two membrane fractions. Original F1-2 preparation/reinjection meaning and biological n remain unresolved.
Experimental metadata notes Sodium-lactate-treated HCT116 cells (25 mM, 48 h); subcellular and whole-cell lactylome coverage. No untreated control among selected files.