KlaAtlas ยท Dataset details

PXD055230

Infection-induced lysine lactylation enables herpesvirus immune evasion

Homo sapiens;Human cytomegalovirus PRIDE 2025/01
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N/A
Reidentified Kla sites
N/A
Reidentified Kla proteins
15
Raw MS files

Dataset overview

Dataset accession
PXD055230
Repository
PRIDE
Publication
39772686
UniProt taxonomy ID
9606;10359
Sample category
Virus-infected cell
Tissue / cell type
Human MRC-5 lung fibroblasts
Experimental condition / comparison
HCMV TB40/E versus mock; infected samples at 24, 48, 72 and 96 h.

Experimental information

Acquisition workflow
Standard
MS platform
Orbitrap
Quantification strategy
Label-free
Kla enrichment method
Anti-lactyllysine antibody-conjugated agarose-bead immunoaffinity purification of peptides
Enrichment reagent supplier
PTM BIO
Reagent catalogue information
Not specified in accessible enrichment protocol

Data access

MaxQuant parameter files
PXD055230_DDA_mqpar.xml, PXD055230_virus_mqpar.xml
Download XML
Raw MS file information
15 processed acquisition files. Original raw data may be accessed through the source repository.
View in PRIDE
Show raw MS file names (15 files)
  • LaIP_TB40_24hpi_DDA_R1.raw
  • LaIP_TB40_24hpi_DDA_R2.raw
  • LaIP_TB40_24hpi_DDA_R3.raw
  • LaIP_TB40_48hpi_DDA_R1.raw
  • LaIP_TB40_48hpi_DDA_R2.raw
  • LaIP_TB40_48hpi_DDA_R3.raw
  • LaIP_TB40_72hpi_DDA_R1.raw
  • LaIP_TB40_72hpi_DDA_R2.raw
  • LaIP_TB40_72hpi_DDA_R3.raw
  • LaIP_TB40_96hpi_DDA_R1.raw
  • LaIP_TB40_96hpi_DDA_R2.raw
  • LaIP_TB40_96hpi_DDA_R3.raw
  • LaIP_TB40_mock_DDA_R1.raw
  • LaIP_TB40_mock_DDA_R2.raw
  • LaIP_TB40_mock_DDA_R3.raw
Metadata sources
https://doi.org/10.1126/sciadv.ads6215
https://www.ebi.ac.uk/pride/archive/projects/PXD055230
Methods: cell infection, lactyl-peptide enrichment and sample preparation
Dataset notes
Sample information and limitations Three biological replicates per condition: mock and four infection time points, 15 unique raw acquisitions. Each prepared sample used six to eight culture dishes. R1-3 denote biological replicates; human/virus XML searches reuse these raws. In the current k_site_positive-derived time analysis, intensities were not normalized to total-proteome abundance; time effects are exploratory lactylation-evidence changes, not occupancy changes.
Experimental metadata notes HCMV TB40/E versus mock; infected samples at 24, 48, 72 and 96 h. Selected files cover the HCMV series.